cd27 pe-cy7 clone# o323 antibody (Thermo Fisher)
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cd27 pe-cy7 clone# o323 antibody
Cd27 Pe Cy7 Clone# O323 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd27+pe-cy7+(clone+o323)/cd27+antibody/pm40412376-192-44-48
Average 90 stars, based on 1 article reviews
Cd27 Pe Cy7 Clone# O323 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd27+pe-cy7+(clone+o323)/cd27+antibody/pm40412376-192-44-48
Average 90 stars, based on 1 article reviews
cd27 pe-cy7 clone# o323 antibody - by Bioz Stars,
2026-09
90/100 stars
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Virus:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Recombinant:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Luciferase:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Mutagenesis:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Plasmid Preparation:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Transfection:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Blocking Assay:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Staining:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Saline:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Immunocytochemistry:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Flow Cytometry:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Expressing:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Negative Control:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Real-time Polymerase Chain Reaction:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Software:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Generated:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: Two Tailed Test:Article Title: Expression of S100A8 protein on B cells is associated with disease activity in patients with systemic lupus erythematosus Article Snippet: To detect S100A8-positive cells, whole blood of HC and patients with SLE were stained with specific antibodies against CD19-V500 (clone HIB19, BD Biosciences, Franklin Lakes, NJ, USA), Article Title: Phenotypic, Genomic and Functional Characterization Reveals No Differences between CD138 ++ and CD138 low Subpopulations in Multiple Myeloma Cell Lines Article Snippet: The origin of the antibodies used in immunocytochemistry and flow cytometry was as follows: anti-CD138-APC (clone B-B4), used for immunocytochemistry and flow cytometry, from Miltenyi Biotec (Auburn, CA); anti-CD20-FITC (clone L27), anti-CD138-PerCP-Cy5 (clone MI15), anti-CD56-APC (clone NCAM16.2), anti-CD45-AmCyan (clone 2D1) and anti-CD38-PE (clone HB7) from BD Biosciences (San Jose, CA, USA); anti-CD19-PacificBlue (clone HIB19) and Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: Cells were labeled with optimized concentrations of the following fluorochrome-mAb conjugates: PerCP-Cy5.5 antihuman IgM (clone G20-127, BD Biosciences, Franklin Lakes, New Jersey), PE anti-human IgD (clone IA6-2, BD Biosciences), PECF594 anti-human CD10 (clone HI10A, BD Biosciences), PE-Cy5 anti-human CD3 (HIT3a, BD Biosciences), PE-Cy5 anti-human CD235a (clone GA-R2, BD Biosciences), Article Title: Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody. Article Snippet: Article Acquisition of quaternary trimer interaction as a key step in the lineage maturation of a broad and potent HIV-1 neutralizing antibody Graphical abstract Highlights • Antibody CH103 makes quaternary contact with the HIV-1 envelope • The quaternary contact was progressively acquired during lineage maturation of CH103 • Two acidic FRH3 residues are critical for this functionally relevant quaternary contact • CH103 can reach two gp120 protomers owing to its unique angle of engagement Authors Qingbo Liu, Ruth J. Parsons, Kevin Wiehe, ..., Barton F. Haynes, Priyamvada Acharya, Paolo Lusso Correspondence liu_qingbo@outlook.com (Q.L.), barton.haynes@duke.edu (B.F.H.), priyamvada.acharya@duke.edu (P.A.), plusso@niaid.nih.gov (P.L.). In brief Liu et al. identify a neutralizing antibody against HIV-1, designated CH103, that binds simultaneously to two HIV-1 envelope subunits and they illustrate the structural basis for such quaternary interaction.. The authors show that this unusual feature was acquired during the antibody maturation path, progressively increasing the potency of the precursor forms. Article Title: Vaccine induction of heterologous HIV-1-neutralizing antibody B cell lineages in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PerCP-Cy5.5 anti-human IgM, Clone G20-127 BD Biosciences Cat#561285; RRID:AB_10611998 PE anti-human IgD, Clone IA6-2 BD Biosciences Cat# 555779; RRID:AB_396114 PE-CF594 anti-human CD10, Clone HI10A BD Biosciences Cat# 562396; RRID:AB_11154416 PE-Cy5 anti-human CD3, Clone HIT3a BD Biosciences Cat# 555341; RRID:AB_395747 PE-Cy5 anti-human CD235a, Clone GA-R2 BD Biosciences Cat# 559944; RRID: AB_397387 PE-Cy7 anti-human CD27, Clone Article Title: A Phase I Trial of SYK Inhibition with Fostamatinib in the Prevention and Treatment of Chronic Graft-Versus-Host Disease. Article Snippet: Upon Fc receptor blocking (TruStain FcX; BioLegend, San Diego, CA), surface staining was performed for 30 minutes on ice in phosphate-buffered saline solution containing 2% fetal bovine serum using the following antibodies: |
